Endocrine Abstracts

Two estrogen receptor isoforms are known to exist, ERα and ERβ. The expression of ER isoforms in breast cancer cell lines was studied to see whether both ERs, as well as any other variants, are expressed at the mRNA and protein level. Three breast cell lines: two which are known to be ER+ve (MCF7 and T47D) and one reported as ER-ve (MDA-MB231) were used in this experiment. For gene expression studies RT-PCR methodology was applied; primers were used that detect ER&#9...